Journal: eLife

Article Title: TDP-43 maximizes nerve conduction velocity by repressing a cryptic exon for paranodal junction assembly in Schwann cells

doi: 10.7554/eLife.64456

Figure Lengend Snippet: ( A ) Immunostaining of Cajal bands in teased P29 ventral roots with an antibody against βII spectrin. ( B ) Immunostaining of teased P29 ventral roots for ankyrinG (AnkG) (red, node) and Kv1.1 channels (white). Localization of Kv1.1 channels in the juxtamesaxon is indicated by open arrowheads. ( C ) Immunostaining of P28 sciatic nerves for zonula occludens-1 (ZO-1) (magenta) and βIV spectrin (green, node). ( D ) Immunostaining of P28 trigeminal nerves for connexin 32 (Cx32) (magenta) and neurofascin (NFasc) (green). ( E ) Immunostaining of P28 sciatic nerves for E-cadherin (E-cad) (red), myelin-associated glycoprotein (MAG) (blue), and NFasc (green). ( F ) Immunostaining of teased P28 sciatic nerves for MAG (green, incisure) and NFasc (magenta). cHet and cKO by Dhh-Cre ( A–F ). Scale bars, 5 μm ( A–F ).

Article Snippet: The following primary antibodies were used: mouse anti-actin (MilliporeSigma, C4), mouse anti-AnkB (UC Davis/NIH NeuroMab Facility, N105/17), mouse anti-AnkG (NeuroMab, N106/36), mouse anti-AnkG (NeuroMab, N106/65), mouse anti-βII spectrin (BD Biosciences, 42), chicken anti-βIV spectrin (a gift from Dr. Matthew N. Rasband), rabbit anti-βIV spectrin (a gift from Dr. Matthew N. Rasband, Baylor College of Medicine), mouse anti-Caspr (Mab275) , rabbit anti-Caspr , rabbit anti-Caspr (Abcam ab34151), rabbit anti-Caspr2 , goat anti-Cntn (R&D Systems, AF904), mouse anti-Cx32 (Thermo Fisher Scientific, 5F9A9), mouse anti-E-cadherin (BD Biosciences, 36), mouse anti-Gldn (Mab94) , rabbit anti-Gldn , rabbit anti-Krox20 (a gift from Dr.

Techniques: Immunostaining

Journal: eLife

Article Title: TDP-43 maximizes nerve conduction velocity by repressing a cryptic exon for paranodal junction assembly in Schwann cells

doi: 10.7554/eLife.64456

Figure Lengend Snippet:

Article Snippet: The following primary antibodies were used: mouse anti-actin (MilliporeSigma, C4), mouse anti-AnkB (UC Davis/NIH NeuroMab Facility, N105/17), mouse anti-AnkG (NeuroMab, N106/36), mouse anti-AnkG (NeuroMab, N106/65), mouse anti-βII spectrin (BD Biosciences, 42), chicken anti-βIV spectrin (a gift from Dr. Matthew N. Rasband), rabbit anti-βIV spectrin (a gift from Dr. Matthew N. Rasband, Baylor College of Medicine), mouse anti-Caspr (Mab275) , rabbit anti-Caspr , rabbit anti-Caspr (Abcam ab34151), rabbit anti-Caspr2 , goat anti-Cntn (R&D Systems, AF904), mouse anti-Cx32 (Thermo Fisher Scientific, 5F9A9), mouse anti-E-cadherin (BD Biosciences, 36), mouse anti-Gldn (Mab94) , rabbit anti-Gldn , rabbit anti-Krox20 (a gift from Dr.

Techniques: Staining

Journal: eLife

Article Title: TDP-43 maximizes nerve conduction velocity by repressing a cryptic exon for paranodal junction assembly in Schwann cells

doi: 10.7554/eLife.64456

Figure Lengend Snippet:

Article Snippet: Antibody , Anti-Cx32 (mouse monoclonal 5F9A9) , Thermo Fisher Scientific , Cat #: 35-8900; RRID: AB_2533228 , (1:200).

Techniques: Staining

Journal: Medicina Oral, Patología Oral y Cirugía Bucal

Article Title: All-trans retinoic acid restores gap junctional intercellular communication between oral cancer cells with upregulation of Cx32 and Cx43 expressions in vitro

doi: 10.4317/medoral.18693

Figure Lengend Snippet: Western blot and immunofluorescence analysis of Cx32, Cx43 and Cx45 protein expression in OSCC cells after ATRA treatment. The expressions of Cx32 (A) and Cx43 (B) significantly decreased in SCC9 and Tca8113 cells as compared with human normal oral epithelial (NOE) cells. After ATRA treatment for 48h, the protein expressions of Cx32 (A) and Cx43 (B) increased in both SCC9 and Tca8113 cells. (C) Compared with human normal oral epithelial (NOE) cells, the protein expression of Cx45 also decreased in SCC9 and Tca8113 cells. However, ATRA treatment did not changed the protein level of Cx45 in SCC9 and Tca8113 cells. (D)ATRA treatment significantly increased the abundance of Cx32 and Cx43 protein and improved localization of fluorescent spots on the plasma membrane of treated SCC9 and Tca8113 cells, compared with those of controls, where the fluorescence appears scattered in the cytoplasm. #P<0.01 relative to normal oral epithelial cells; *P<0.05 relative to ethanol control. Bar: 20μМ.

Article Snippet: Cells were incubated overnight at 4°C in 1:100 mouse anti-connexin 43 (Invitrogen) or 1:50 mouse anti-Cx32 antibody (Invitrogen).

Techniques: Western Blot, Immunofluorescence, Expressing, Fluorescence

Journal: Medicina Oral, Patología Oral y Cirugía Bucal

Article Title: All-trans retinoic acid restores gap junctional intercellular communication between oral cancer cells with upregulation of Cx32 and Cx43 expressions in vitro

doi: 10.4317/medoral.18693

Figure Lengend Snippet: Western blot and immunofluorescence analysis of Cx32, Cx43 and Cx45 protein expression in OSCC cells after ATRA treatment. The expressions of Cx32 (A) and Cx43 (B) significantly decreased in SCC9 and Tca8113 cells as compared with human normal oral epithelial (NOE) cells. After ATRA treatment for 48h, the protein expressions of Cx32 (A) and Cx43 (B) increased in both SCC9 and Tca8113 cells. (C) Compared with human normal oral epithelial (NOE) cells, the protein expression of Cx45 also decreased in SCC9 and Tca8113 cells. However, ATRA treatment did not changed the protein level of Cx45 in SCC9 and Tca8113 cells. (D)ATRA treatment significantly increased the abundance of Cx32 and Cx43 protein and improved localization of fluorescent spots on the plasma membrane of treated SCC9 and Tca8113 cells, compared with those of controls, where the fluorescence appears scattered in the cytoplasm. #P<0.01 relative to normal oral epithelial cells; *P<0.05 relative to ethanol control. Bar: 20μМ.

Article Snippet: Equal amounts of protein were loaded for SDS-PAGE and immunoblotted with the following primary antibodies: mouse monoclonal anti-Cx32 (Invitrogen; 1:800), mouse monoclonal anti-Cx43 (Invitrogen; 1:800), mouse monoclonal anti-Cx45 (Millipore, Billerica, Massachusetts, USA; 1:500), and glyceralde-hyde-3-phosphate dehydrogenase (GAPDH, Invitrogen).

Techniques: Western Blot, Immunofluorescence, Expressing, Fluorescence